Nikon Upright Microscope · MicroCosmos

Nikon ECLIPSE Si Upright Microscope

Name: Nikon ECLIPSE Si Upright Microscope
Brand: Nikon
SKU: ECLIPSE-Si
Rating: 4.7 (9 reviews)

Focus in comfort. Comfort in focus.

An ergonomically engineered clinical and laboratory microscope built for professionals who spend hours at the eyepiece — with intelligent auto-brightness, a low-strain stage, and Nikon's renowned CFI60 optics.

CFI60 Infinity Optics LIM Auto Light Intensity 135 mm Low Stage 5 Observation Methods

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What is the Nikon ECLIPSE Si?

The Nikon ECLIPSE Si is an ergonomically designed upright microscope for clinical, pathology, and life-science laboratories. It reduces operator fatigue with a low 135 mm stage, a 45° eyepiece tube inclination, and one-handed coarse/fine focus plus stage control. Its intelligent Light Intensity Management (LIM) system automatically remembers ideal LED brightness per objective, cutting light-adjustment time by up to 40%. Built on Nikon's CFI60 infinity optical system with CFI E Plan objectives, the ECLIPSE Si supports five observation methods — brightfield, phase contrast, darkfield, simple polarizing, and diascopic fluorescence — and is compatible with Digital Sight cameras for PC-free image sharing.

Key Features

Pursuing Efficient Workflow

The ECLIPSE Si eliminates unnecessary adjustments and enables efficient, comfortable operation — with an ergonomic design that supports natural posture, even during repetitive high-volume slide review.

LIM Feature Control

Push-Button Brightness Memory

Press the brightness knob to toggle LIM ON/OFF
Rotate to set brightness for the active objective
Setting is remembered automatically per objective
Status shown live on the built-in LCD display

Maintaining Comfortable Brightness When Switching Magnifications

Every objective transmits light differently, so brightness must be re-adjusted each time you change magnification — and sudden jumps from high to low power often cause eye strain. The ECLIPSE Si's intelligent Light Intensity Management (LIM) automatically remembers and restores the correct light intensity for each objective, cutting time spent adjusting brightness by up to 40%.*

*Compared to switching among three objectives on a previous LED-based Nikon model, tested by Nikon.

LIM TechnologyEye-Strain Reduction

LIM feature OFF with a high-powered objective — brightness appears mismatched — LIM OFF — High-Power Objective

LIM feature OFF with a low-powered objective — brightness appears mismatched — LIM OFF — Low-Power Objective

LIM feature ON with a high-powered objective — brightness automatically optimised — LIM ON — High-Power Objective

LIM feature ON with a low-powered objective — brightness automatically optimised — LIM ON — Low-Power Objective

Close-up of the easy-to-operate specimen holder lever on the ECLIPSE Si low-height stage

Low Stage for Effortless Slide Replacement

The ECLIPSE Si stage sits at just 135 mm — about 50 mm lower than Nikon's conventional upright microscopes — shrinking the range of motion needed to exchange slides and reducing arm/shoulder fatigue. The stage movement knob sits lower too, so you can explore different areas of a slide while resting your hands on the table. The specimen-holder lever is shaped for easy, ergonomic operation, and the stage footprint itself is 30% smaller than conventional designs.

Ergonomic Stage30% Smaller Footprint

Side-by-side comparison of a conventional Nikon microscope beside the ECLIPSE Si showing the lower stage height difference — Conventional Microscope vs ECLIPSE Si Stage Height

Close-up detail of the coarse focus knob and fine focus knob on the ECLIPSE Si — Coarse Focus Knob & Fine Focus Knob

Optional eye-level riser accessory mounted beneath the eyepiece tube to raise eyepoint height

Enables Natural Posture Throughout the Workflow

The eyepiece tube's 45° inclination angle lets you observe specimens while keeping a natural, upright posture. Combined with the low stage design, users can move seamlessly between looking through the eyepieces and checking slide placement — without adjusting posture. An optional eye-level riser raises the eyepoint by a further 25 mm (up to two can be stacked for 50 mm) to tailor the height to each observer.

45° Tube InclinationPosture Comfort

Diagram showing the ECLIPSE Si vertical stop stage-height stopper screw being turned to set the upper focus limit

Worry-Free Focusing Thanks to Vertical Stop

A built-in stopper lets you set the upper limit of stage travel. Once set, the stage will not rise past that height even if the focus knob keeps turning — eliminating the risk of over-focusing, which can break slides or damage objectives. Exchange specimens and focus with total confidence.

Slide ProtectionObjective Protection

Diagram confirming the ECLIPSE Si stage does not rise above the pre-set vertical stop height

Key Features

Pursuing Stress-Free Ease of Use

Innovative features and intelligent design minimize unnecessary body movement, saving time and reducing strain — even when examining large numbers of slides.

Demonstration of coarse/fine focus and stage movement being operated with one hand on the ECLIPSE Si

Adjusting Focus & Moving the Stage With One Hand

Coarse and fine focus knobs sit on both sides of the microscope for right- or left-hand focusing. The stage handle sits close to the focus knob so one hand can control both focus and stage position — freeing the other hand to rotate the nosepiece or swap slides. This delivers an efficient workflow even through a large slide caseload.

Ambidextrous FocusHigh-Throughput Ready

ECLIPSE Si LCD status display showing ECO mode, LIM function, objective name, magnification and brightness bar graph

Status Display & Knob Rotation Direction Display

Illumination brightness appears as a bar graph on the built-in LCD, so you can check magnification at a glance while maintaining your observation posture. The status display shows five pieces of information simultaneously:

❶ECO mode: ON / OFF status
❷LIM function: ON / OFF status
❸Objective name — currently active lens
❹Magnification — current objective power
❺Brightness state — LED intensity bar graph

The rotation direction of the focus and brightness knobs is also displayed intuitively, and the stage-movement knob icon is illustrated on the specimen travel scale so there's no hesitation about which control to use.

At-a-Glance LCDIntuitive Controls

The direction of rotation of the focus and brightness control knobs is intuitively indicated on the body, and the icon of the stage-movement knob to be operated is illustrated on the forward-backward and right-left travel scale — removing any hesitation about which control to use next.

Diagram showing the direction of rotation indicator on the ECLIPSE Si focus and brightness knobs

Close-up of the easy-to-rotate quintuple reversed-type nosepiece on the ECLIPSE Si accommodating five objectives

Changing Magnifications Comfortably

The reversed-type nosepiece gives easy access and visibility to the objective in use, and sits low on the body to reduce arm strain during frequent magnification changes. Its easy-grip design enables smooth rotation and accommodates up to five objectives for a wide magnification range — plus an optional blue-light-blocking filter and automatic ECO-mode shutdown after inactivity to save power effortlessly.

5-Position NosepieceECO Mode

E2-F-BF Blue Light Blocking Filter — a 45mm optional filter that fits the illuminator filter slot to reduce blue-light glare during long observation sessions.

Digital Imaging

Mount a digital camera on the ECLIPSE Si trinocular tube to display and record specimen images without a PC.

Simultaneous Viewing

One Specimen, Many Viewers

Connect Digital Sight 1000/100 via HDMI to any monitor
Whole team views the live specimen image at once
No PC required — capture directly to the camera
Ideal for training, second opinions, and case review

Attach the Digital Sight 1000 color microscope camera to display specimen images on a monitor for simultaneous multi-person observation, and record images without a PC. The higher-resolution Digital Sight 100 camera offers superior color reproduction and high-resolution capture. NIS-Elements LE imaging software is required.

PC-Free CaptureHDMI Monitor Output

Real-time sharing of specimen images between PCs in different rooms or buildings using ECLIPSE Si connected imaging

Optical Performance

Superior Optics for High-Quality Images

Nikon's advanced optical technology — refined across a century of microscope manufacturing — powers every ECLIPSE Si observation.

CFI E Plan series objective lenses mounted on the ECLIPSE Si nosepiece

Excellent Image Flatness & Chromatic Aberration Correction

The ECLIPSE Si employs CFI E Plan series objectives, delivering flat, sharp images to the periphery of the field of view. These are part of the CFI60 infinity-corrected optical system, achieving both high resolution and long working distances — with a wide range of Nikon CFI60 objectives compatible.

CFI60 Infinity SystemFlat-Field Optics

22mm Field of View

When equipped with FOV22 tubes (C-TB, C-TF, C-TT, C-TE2) and lenses, the ECLIPSE Si delivers a large 22mm field of view for more efficient clinical observation.

Long-Life LED, Constant Colour Temperature

The high-intensity white LED lasts up to 60,000 hours. Since colour temperature stays constant as brightness changes, image colour doesn't shift when switching magnifications.

Uniform Field Brightness

An integrated fly-eye lens in the illumination system provides uniform brightness across the entire field of view, edge to edge.

Field Diaphragm Control

A field diaphragm limits the illumination range to suppress flare and ghosting for high-contrast images, and limits photobleaching during fluorescence work.

Versatile Optics

Compatible With Five Observation Methods

Using optional accessories, the ECLIPSE Si supports brightfield plus four additional observation techniques for broader diagnostic and research versatility.

Brightfield Observation

High-quality images with bright, uniform illumination across the full field of view, using objectives with superior flatness and chromatic correction. Standard on every ECLIPSE Si — no extra accessories required.

Phase Contrast Observation

A phase condenser with CFI DL phase-contrast objectives (or a standard Abbe condenser with a PH slider) reveals colourless, transparent specimens in high contrast without staining or labeling.

Requires: CFI DL phase contrast objectives, GIF filter, centering telescope, and either phase contrast sliders (set of 2) or a phase condenser with height-adjustment tool.

Darkfield Observation

Inserting a darkfield slider into the condenser slot uses oblique illumination to reveal light scattered by unstained specimens such as live bacteria or colloidal particles.

Note: Not compatible with the CFI E Plan Achromat 4X objective.

Simple Polarizing Observation

Attach a polarizer to the field lens and an analyzer to the eyepiece tube mount; rotate the polarizer to adjust the polarization state for birefringence studies.

Diascopic Fluorescence Observation

Nikon's unique diascopic fluorescence method needs no dedicated episcopic illuminator — simply insert an EX filter slider into the condenser slot and a BA filter slider into the nosepiece slot to observe specimens expressing GFP or stained with FITC or Alexa 488-class dyes.

Portability

Emphasis on Easy Storage

The ECLIPSE Si is lightweight, easy to carry, and designed to store neatly after use.

Easy to Carry, Easy to Store

The ECLIPSE Si body is 14% lighter than the previous LED model. Grips on both sides of the base and rear arms make carrying stable and secure, and eyepieces can be screw-locked to prevent them falling during transport. For storage, the tube rotates backward (after loosening the tube-locking screw) to reduce footprint — it's engineered not to fall even when loosened — and the AC adaptor and power cord stow neatly at the rear of the unit.

14% Lighter BodyCompact Storage

Storage Configuration

Backward Tube Rotation

Tube rotates backward after loosening the tube-locking screw
Engineered so the tube will not fall even when the screw is loose
Rotation direction is clearly illustrated on the microscope body
AC adaptor stores at the rear; power cord winds up neatly

Support

Online Guide Accessible by Smartphone

Scan the QR code sticker on the microscope to open a web-based operation manual with step-by-step videos covering setup and use.

Nikon ECLIPSE Si online operation guide displayed and accessible on a smartphone via QR code

QR-Linked Manual

Scan. Watch. Set Up.

A QR code sticker on the microscope body links directly to Nikon's web-based Online Guide — complete with instructional videos covering initial setup, focusing, LIM activation, and observation-method changeovers, viewable on any smartphone.

Technical Data

Full Specifications

Complete technical specifications for the Nikon ECLIPSE Si upright microscope, as published in the official Nikon ECLIPSE Si brochure.

Main Body
Optical system	CFI60 Infinity optical system
Illumination	High-luminescent white LED illuminator (Eco-illumination); built-in fly-eye lens; up to two 45 mm diameter filters can be installed*¹; Light Intensity Management (LIM) feature included
Focusing	Coaxial coarse/fine focusing (both sides), cross roller guide. Focusing stroke: Up 2 mm / Down 13 mm. Coarse: 37.7 mm per rotation. Fine: 0.2 mm per rotation. Minimum reading: 2 µm. Includes coarse-focus torque adjustment ring and stage vertical-movement stopper (vertical stop)

Optics, Stage & Accessories
Eyepieces (F.O.V., mm)	With diopter adjustment — E2-CFI 10X (20)², E2-CFI 15X (12)², CFI 10X (22)*³
Tubes	Inclination 45°, pupillary distance 50–75 mm, 2-position eyepoint height: EC-T-TB2 Binocular Tube 2 / EC-T-TF2 Trinocular Tube F2 (Eyepiece:Port 50:50, built-in 0.55X relay lens, C-mount) Inclination 25°, pupillary distance 50–75 mm: C-TB Binocular Tube / C-TF Trinocular Tube F (100:0, 0:100) / C-TT Trinocular Tube T (100:0, 20:80, 0:100) Inclination 10°–30°, extension up to 40 mm: C-TE2 Ergonomic Binocular Tube (100:0, 50:50 via optional C-TEP3 DSC Port C-0.55X)
Nosepiece	Reversed-type quintuple nosepiece (within main body)
Stage	Rectangular mechanical stage (within main body) with specimen holder 2L and vernier calibrations; cross travel 76 (X) × 52 (Y) mm; stage height 135 mm
Objectives (NA / W.D.)	CFI E Plan Achromat 4X (0.1/30mm)⁴ · CFI E Plan Achromat 10X (0.25/7mm) · CFI E Plan Achromat 40X (0.65/0.65mm) · CFI E Plan Achromat 60X (0.8/0.3mm)⁴ · CFI E Plan Achromat 100X Oil (1.25/0.23mm)*⁴ Phase contrast objectives: CFI Achromat DL 10X (0.25/7.0mm) · CFI Achromat LWD DL 20X (0.40/3.90mm) · CFI Achromat DL 40X (0.65/0.65mm) · CFI Achromat DL 100X Oil (1.25/0.23mm) Other CFI60 objectives also compatible.
Condenser	Abbe Condenser, NA 1.25, vertically movable and centerable
Observation methods*⁵	Brightfield, phase contrast, diascopic fluorescence, darkfield, simple polarizing
Fungus-proof treatment	Antifungal paint applied around the optical system
Optional accessories	E2-SPH1 10X–40X PH slider / E2-SPH2 100X PH slider (phase contrast objectives) · Phase Condenser⁶ · E2-F-FL Dia-FL set GFP-B · E2-SDF darkfield slider · E2-DP simple polarizer / E2-PSA simple analyzer · EC-ER eye-level riser · E2-F-BF blue light blocking filter · Object marker⁶*⁷ · Eyepiece pointer · Teaching head
Power supply	Included AC adapter — input: 100–240 VAC, 0.48A max., 50–60 Hz; output: 5.0 VDC, 3.0A max.
Power consumption (max.)	Nominal value: 5 W
Weight (approx.)	Approx. 6.0 kg (binocular tube) · Approx. 6.4 kg (trinocular tube)

*1 If thickness is 2.5 mm or less; only one filter when a simple polarizer is attached · *2 Used with EC-T-TB2 / EC-T-TF2 tubes · *3 Used with C-TB, C-TF/C-TT, or C-TE2 tubes · *4 Not usable for darkfield observation · *5 Observations other than brightfield require optional accessories · *6 Not for medical purposes · *7 C-OA 15mm adapter required.
Digital Sight 1000, DS-Fi3 camera, and NIS-Elements L Imaging software are not for medical purposes. Specifications subject to change without notice.

Optional Accessories

Expand the ECLIPSE Si to Fit Your Workflow

Purpose-built accessories extend the ECLIPSE Si for digital imaging, teaching, ergonomic tailoring, and specialised observation.

Nikon Digital Sight 1000 color microscope camera mounted on ECLIPSE Si trinocular tube

Digital Sight 1000 Microscope Camera

2-megapixel CMOS sensor; captures color images and video up to 1920×1080. Connect a monitor via HDMI and a mouse for PC-free image capture and simple point-to-point measurements.

Nikon Digital Sight 100 microscope digital camera, wide product shot

Digital Sight 100 Microscope Camera

17.7-megapixel CMOS sensor with 4K image capture, high-speed live display, and PC-free observation via HDMI monitor connection.

NIS-Elements LE microscope imaging software interface on desktop PC screen

NIS-Elements LE Imaging Software

Free software for configuring and controlling microscope digital cameras, displaying live images, and capturing images. Installs on desktop or tablet PCs (Windows 11 Pro 64-bit supported); contact MicroCosmos for PC specification guidance.

Teaching Head

Enables simultaneous two-person observation on one microscope. Available in face-to-face and side-by-side types, with a built-in LED pointer to indicate areas of interest to a second observer.

Eye-Level Riser

Mounts under the eyepiece tube to raise the eyepoint by 25 mm; up to two pieces (50 mm total) can be stacked to fit each observer for a comfortable viewing posture.

Why MicroCosmos Recommends It

ECLIPSE Si at a Glance

A quick recap of what sets the Nikon ECLIPSE Si apart as an ergonomic upright microscope for clinical, pathology, cytology, and life-science laboratories.

Ergonomics-First Engineering

135 mm low stage, 45° tube inclination, one-handed focus/stage control, and a 30% smaller stage footprint reduce fatigue across full-day slide review sessions.

Intelligent Automation

LIM auto-brightness memory, ECO auto-shutdown, and an at-a-glance LCD status display remove manual guesswork from everyday operation.

Nikon Optical Heritage

CFI60 infinity optics with CFI E Plan objectives deliver flat, chromatically corrected images backed by a century of Nikon microscope engineering.

Five Observation Methods

Brightfield, phase contrast, darkfield, simple polarizing, and diascopic fluorescence — all on a single, compact upright platform.

Digital-Ready Imaging

Trinocular tube support for Digital Sight 1000 / 100 cameras enables PC-free, monitor-based image sharing on-site and across rooms or buildings.

Light, Portable, Storage-Smart

14% lighter than the previous LED model, with a backward-folding tube and tidy cable/adaptor storage for labs with limited bench space.

FAQ

Frequently Asked Questions

Common questions about the Nikon ECLIPSE Si upright microscope, answered directly.

What is the Nikon ECLIPSE Si microscope?

The Nikon ECLIPSE Si is an ergonomically engineered upright microscope designed for professionals who spend long hours at the eyepiece. It reduces physical strain through a low 135 mm stage, a 45-degree eyepiece inclination, one-handed focus and stage control, and an intelligent Light Intensity Management (LIM) system that automatically recalls brightness settings per objective.

What is Nikon's LIM (Light Intensity Management) feature?

LIM is an intelligent brightness-memory system that automatically stores and restores the ideal LED light intensity for each objective lens. It removes the need to manually readjust brightness every time the magnification is changed, cutting light-adjustment time by up to 40% and reducing eye strain from sudden brightness jumps.

Which observation methods does the ECLIPSE Si support?

With the appropriate optional accessories, the ECLIPSE Si supports five observation methods: brightfield, phase contrast, darkfield, simple polarizing, and diascopic fluorescence (for GFP, FITC, and Alexa 488-class dyes) — without a dedicated episcopic fluorescence illuminator.

What objectives and optical system does the ECLIPSE Si use?

It uses Nikon's CFI60 infinity-corrected optical system with CFI E Plan Achromat objectives (4X, 10X, 40X, 60X, 100X oil) delivering flat, sharp fields of view with excellent chromatic aberration correction and long working distances. It's also compatible with CFI DL phase-contrast objectives and other CFI60 lenses.

Why is the ECLIPSE Si stage lower than conventional microscopes?

The stage sits at 135 mm, about 50 mm lower than Nikon's conventional upright microscopes. The lower stage and lower stage-movement knob shrink the motion needed to change slides, reducing arm and shoulder fatigue during high-throughput slide review.

What is the vertical stop feature on the ECLIPSE Si?

The vertical stop is a stage-height stopper that lets users set an upper focusing limit. Once set, the stage cannot rise above that height even if the focus knob keeps turning — preventing accidental slide breakage or objective damage from over-focusing.

Can a camera be attached to the ECLIPSE Si?

Yes. The trinocular tube accepts the Nikon Digital Sight 1000 (2-megapixel, PC-free HDMI capture) or Digital Sight 100 (17.7-megapixel, 4K capture) cameras, letting users display and record specimen images on a monitor without a PC, using NIS-Elements LE software.

What optional accessories are available for the ECLIPSE Si?

Digital Sight 1000 and Digital Sight 100 cameras, NIS-Elements LE software, a teaching head for dual observation, an eye-level riser (+25 mm eyepoint), a blue-light-blocking filter, phase contrast sliders and condenser, a darkfield slider, simple polarizer/analyzer, and a diascopic fluorescence filter set.

Bring the ECLIPSE Si Into Your Lab

MicroCosmos is your authorized source for the Nikon ECLIPSE Si upright microscope. Talk to our team for pricing, a live demo, or help configuring the right observation methods and accessories for your workflow.

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